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Two-VHH CAR

/two-vhh

TL;DR

Tandem/dual-VHH CAR concept surface. Use for architecture planning, then inspect isolated-pair pose signals in CAR Interface Assessment and verify binding experimentally.

Use it / Skip it

This is a specialized design surface that remains available for project-specific work, but it is no longer the main platform narrative. Use the Workspace modules for daily intake, triage, scoring, and review.

Use when

A project specifically needs this format, constraint, or legacy flow and the responsible owner has confirmed the backend path for the run.

Don't use for

Do not use it as the default antibody discovery path. For current production work, start from NGS Pool, Semi-de-novo, Interface, Affinity, RFantibody, or CAR Interface Assessment.

Inputs

Target or parent
The antigen, parent binder, construct context, or structural constraint required by the page.
Design settings
Format-specific parameters such as epitope, framework, count, construct components, linker, or backend.
Owner confirmation
For partial/WIP pages, confirm the execution path before promising a wet-lab handoff.

Outputs

Configuration
A structured record of the design intent and selected parameters.
Run or preview
Available only where the backend is wired; otherwise treat the page as planning UI.
Handoff note
The next module or manual review needed before candidate decisions.

Walkthrough

  1. 1. Confirm this specialized page is the right entry point for the project

    Confirm this specialized page is the right entry point for the project.

  2. 2. Fill only the parameters that are meaningful for the current biological question

    Fill only the parameters that are meaningful for the current biological question.

  3. 3. Check whether the backend is wired before interpreting a submitted run as real work

    Check whether the backend is wired before interpreting a submitted run as real work.

  4. 4. Move promising sequences or structures back to Interface, Affinity, Compare, or CAR Interface Assessment for evidence review

    Move promising sequences or structures back to Interface, Affinity, Compare, or CAR Interface Assessment for evidence review.

Under the hood

  • Backend status varies by specialized module.
  • Some pages have UI and API stubs ahead of full orchestration.
  • Current production evidence should be anchored in persisted jobs and exported artifacts.

Worked example

Specialized design review
Use the page to capture the design setup, run only if the backend is confirmed, then route any generated sequence or structure through Interface and Affinity before wet-lab discussion.

Pitfalls

  • A visible form is not proof that the end-to-end backend is production ready.
  • Do not bypass evidence review just because a specialized module produced an attractive preview.
  • Keep the module owner in the loop for WIP or partial flows.

See also