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CAR Design

/car-design

TL;DR

A WIP cassette-planning surface for binder, hinge, TM, costim, and CD3z choices.

Use it / Skip it

The page keeps CAR construct thinking visible, but candidate handoff and wet-lab-grade cassette generation are still being hardened.

Use when

You need to discuss construct architecture and component choices after a binder has been shortlisted.

Don't use for

Do not treat it as the current production path for validated CAR cassette output.

Inputs

Binder
Candidate binder sequence or project context.
Components
Signal peptide, hinge, transmembrane, costim, and CD3z choices.

Outputs

Architecture preview
Construct-level planning view.
Review context
Notes for downstream manual/wet-lab planning.

Walkthrough

  1. 1. Start from a binder that already has Interface/Affinity context

    Start from a binder that already has Interface/Affinity context.

  2. 2. Choose construct components for discussion

    Choose construct components for discussion.

  3. 3. Record caveats and hand off to manual review

    Record caveats and hand off to manual review.

Under the hood

  • Current sidebar marks this as WIP; treat backend handoff as incomplete.

Worked example

Discuss a BCMA binder cassette
Use a shortlisted BCMA binder to compare 4-1BB vs CD28 style decisions, but keep final construct generation outside the page until the handoff is hardened.

Pitfalls

  • Architecture preview is not wet-lab approval.
  • Do not skip CAR Interface or manual construct review.

See also